Sentence examples similar to cells well washed from inspiring English sources

Cells were cultured in 6-well plates at a density of 2 × 105 cells per well, washed and incubated with 10 μM of the STAT-1 decoy ODN or the mutant control ODN.

For determination of the number of adherent cells, wells were washed three times with PBS followed by a 30 min trypsin (porcine, 2.5%, 0.5 g/L; Gibco #27250-018) treatment.

After this time, the culture medium was removed and the cells attached to the wells washed twice with PBS.

After 24 h, cells were discarded and wells washed, followed by use of biotinylated anti-IFN-γ mAb, streptavidin-HRP and substrate reaction.

After incubation at 37°C in culture media and doxorubicin or etoposide at the concentrations indicated for 24 h, cells were harvested from wells, washed with PBS and lysed in lysis buffer, consisting of 1% Brij 97, 5 m M EDTA, 0.02  M HEPES, pH 7.3, 0.15  M NaCl, 1 m M PMSF, 0.5 m M NaF, 10  μg ml−1 aprotinin and 0.2 m M sodium orthovanadate.

Buffer was then removed from each well, cells washed once in HBSS and fresh HBSS added at room temperature.

Wash cells well 2-3x with cold APBS.

After aspirating the medium from each well, cells were washed 5 times with PBS to remove unattached cells and incubated with Cell Stain Solution for 10 min. Following the removal of Cell Stain Solution, cells in each well were again washed 5 times with water and allowed to dry in air.

Cells were washed well with 1% FCS/PBS between incubations.

Cells were washed well with 1 × PBS at room temperature to remove all traces of methyl cellulose and pelleted for chromatin isolation.

The next day, the media was aspirated from each well, cells were washed with phosphate-buffered saline (PBS), and fresh media [DMEM/F-12 containing 10% charcoal/dextran-treated FBS (HyClone, Logan, UT, USA)] was added to each well.