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This indicated that the yeast cells well grew in the entrapment matrix.
HCT116 cells were seeded in another six-well tissue culture plate (Costar) at 1.6 × 10 cells per well, grown to 80% confluence one day before treatment.
Unlike most other types of adult stem cells, mesenchymal cells grow well in culture, so thousands of doses can be produced from a single donation.
Cells (10 cells per well) were grown in 24-well plates and exposed to increasing doses of the drugs.
Two hundred thousand and one hundred thousand HeLa cells per well were grown in 12-well plates, or 24-well plates, respectively in complete medium.
Cells were seeded in 96-well plates at 8000 cells per well and grown for 18 h.
Ovarian cells were plated in 6-well plates at 250,000 cells per well and grown in complete medium.
Melanoma cells were seeded into six-well plates at 10 cells per well and grown until 60% confluent.
Cells were plated onto a 24-well plate at 0.5 × 10 cells per well and grown overnight before the experiment.
The cells were seeded at a density of 24,000 cells per well and grown for 24 hours.
CHO‐745 cells were seeded at a density of 15 000 cells per well and grown for 48 h before assays.
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