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The number of cells was determined using NIS-Elements AR3.0 software (Nikon, Melville, NY).
A gate excluding cell debris and nonviable cells was determined using forward vs. side scatter parameters.
The morphology of free cells was determined using a scanning electron microscope (SEM; S-570, Hitachi, Chiyoda-ku, Japan).
The number of the cells was determined using the image analysis software NIS-Elements (Melville, NY, USA).
The number of unattached cells was determined using a Coulter counter (Beckman Coulter).
The effect of r84 on endothelial cells was determined using several in vitro assays.
Viability of cells was determined using a LIVE/DEAD Fixable Dead Stain Kit (Invitrogen).
The mitochondrial membrane potential of yeast cells was determined using a flow cytometer (FACScan, BD).
DNA content of C. albicans cells was determined using FACS analysis [84].
3-Nitrotyrosine level in cultured cells was determined using the immunocytochemical staining method.
Specificity of IE binding to the BeWo cells was determined using various inhibitors.
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