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A way to eradicate this negative effect is to induce the death of the modified cells upon request including a suicide gene in the gene transfer vector.
The aim of this study was to find a way to induce the death (here by apoptosis) of targeted cells upon request.
We proposed here a way to induce the death of gene modified cells upon request by acting on a pro-apoptotic protein cellular localization and on the activation of its apoptotic function.
These new properties of an ectopic protein could be used to develop a 'gain of function' approach designed to allow the death by apoptosis of targeted gene modified cells upon request.
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Most guests are too focused on the blissful setting to notice the grainy TV reception or absence od phones (cell phones available upon request), let alone where anyone else is bathing.
For downregulation of TFPIβ, cDNAs encoding shRNAs against this isoform were cloned into the pSiRPG vector and transduced into MDA-MB-231 cells (shRNA sequences available upon request).
These arbitrarily named and subsequently characterized cell lines are available upon request.
Results for non-commercial cell lines will be given upon request.
The sequences resulting in the best KD (available upon request) compatible with cell viability were used for further experiments.
The cell lines will be made available upon request for non-commercial use only under the restrictions of an MTA.
Primers sequences for RTPCR (Sigma Genosys, The Woodlands, TX) and detailed description of antibodies for Western blot (Cell Signaling, Danvers, MA) are available upon request.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com