Nearly all of the cells treated with increasing concentrations of AA became necrotic, without an increase in apoptosis.
Cells treated with increasing concentrations of HPB-CD for 2 hours were assessed for cell viability using an MTT assay [13].
LF activity was assessed by examining MEK2 cleavage by Western blotting lysates from cells treated with increasing concentrations of toxin cell lysates with antibodies against MEK2 (from Cell Signaling; Danvers, MA) and ß-actin (Ambion; Austin, TX).
Cellular toxicity of RBV was determined using about 80% confluent cells treated with increasing RBV concentrations (0, 200, 500 or 1000 µM) at 37°C and 5% CO2 for 24 h.
Indeed, immunoblot analysis of cytoplasmic preparations of HEK293 cells treated with increasing concentrations of pyrvinium demonstrated dose-dependent decreased and increased levels of β-catenin and Axin, respectively (Figure 1E and Figure S1A and B).
When cells treated with increasing concentrations of this drug, ranging from 10 µM to 50 µM, were challenged with 37997-GFP CHIKV, the percentage of GFP-expressing cells was found reduced from 52% to 68% when compared with cells maintained in medium supplemented with drug solvent (Figure 5B).
In order to analyze if the changes observed on the RNA level were also mirrored by changes on the protein level, immunoblot analysis of TP53 and CDKN1A were performed from lysates of A549 cells treated with increasing concentrations of SAG or PAC for 18 hrs.
In contrast, yeast cells treated with increasing concentrations of farnesol exhibited extensive cytoplasmic organelles damages.
(D ) Hh reporter activity in cells treated with increasing concentrations of the indicated oxysterols.
(B ) RBM47 mRNA expression measured by quantitative real-time RT-PCR in WT10 cells treated with increasing concentrations of doxycycline.
(C ) RBM47 mRNA expression measured by quantitative real-time RT-PCR in MUT3 cells treated with increasing concentrations of doxycycline.
