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EGFP-expressing plasmid (0.5 µg) was used for co-transfection with pNPM1NS1 and pNPM1M2NS1 to visualize transfected cells (transfection efficiency was 80 90%).
Compared to the mock-transfected cells, transfection with the HBx plasmid augmented the luciferase activity of the SOCS3 promoter over two folds, indicating that the induction of SOCS3 in response to HBx is mediated through the transcriptional activation of the SOCS3 gene.
For one, a larger amount of cDNA not only increases the amount of cDNA per cell, but also the number of transfected cells (transfection efficiency).
All constructs were transfected into MM1 cells (transfection efficiency: 45 50%; not shown), and their activity was assessed, after incubation with either 20 ng/ml IL-6 or culture medium, by using the luciferase reporter assay and the Renila system.
The resulting construct was transfected into tsA-201 cells (transfection details below).
In all tested lung cancer cells, transfection with TS-1 resulted in marked depletion of TS, whereas no such effect was observed in cells transfected with a nonspecific siRNA.
Similarly, tumor cells transfection with Met specific siRNA significantly inhibits HGF-dependent growth [24].
Key to non-invasive imaging is to incorporation of these reporter genes (talked above) in to the cells "Transfection".
The present study was aimed at studying the effects of 2-MHz pulsed Doppler US on malignant brain tumor cells transfection by cationic liposome/plasmid-DNA complexes (lipoplexes).
Primary CD4+ T cells transfection.
Cells transfection was performed with Lipofectamine 2000 according to the manufacturers protocol (Invitrogen).
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