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To evaluate the directionality of cells, trajectory angles of each cell with respect to the X axis were determined at 10 minute intervals.
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Liquid velocity profiles, cells' movement, and the temporal irradiance patterns obtained by coupling the cells' trajectories and the irradiance distribution model have been determined.
Therefore, cell-scaffold interception should be promoted and the present computational model which predicts the effect of gravity and fluid drag on cells trajectories could serve to optimise bioreactors and enhance cell seeding efficiency.
Pseudotemporal ordering applies machine learning methods to scRNA-seq data to reconstruct cells' trajectories as they undergo a dynamic biological process.
In contrast to IP and AP patterns which are symmetrical (cells' trajectories in the phase plane are identical), the AIP pattern is not symmetrical (trajectories of the cells differ) and consists in two cells' active phases expressed with the phase shift Φ<0.5 (see [16]).
Rights & permissionsfor article A comparison of single-cell trajectory inference methods.
Cell trajectory directionality was estimated as the ratio of displacement over trajectory length both in 3D.
Each CR cell trajectory is labelled with a different colour line using the (ImageJ) software analysis.
Bendall, S.C. et al. Single-cell trajectory detection uncovers progression and regulatory coordination in human B cell development.
Cell trajectory acquisition was performed using a hand-coded semi-automatic MATLAB script described in previous studies68,69,70.
Σ, sum of all cell trajectory vectors divided by the number of cells (n = 30 cells, each line represents a cell).
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