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As the VEGF concentration decreases (inside the matrigel, or in the ECM surrounding the muscle fibres) the proliferation stimulus diminishes, permitting these hematopoietic/endothelial precursors cells to then differentiate into muscle fibres.
We hypothesized that the early developing ovary is composed of a cluster of GREL cells, which arise from the mesonephric surface epithelium through proliferation in a process that is also associated with degradation of the basal lamina allowing the primordial germ cells to then associate with GREL cells.
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For northern blot, we grew cells to OD600 ∼0.5, then applied treatment.
"Tumors use EGFL6 to tell the cancer cells to migrate and then divide.
Autophagy is a fundamental process used by cells to first degrade and then re-use cell components - sort of a cellular recycling service.
The growth of a bacterial population occurs in a geometric or exponential manner: with each division cycle (generation), one cell gives rise to 2 cells, then 4 cells, then 8 cells, then 16, then 32, and so forth.
Subsequent actions by PGC1-alpha then stimulate cells to produce more mitochondria.
TER119−CD71− cells differentiate first to TER119−CD71+ cells, subsequently to TER119++CD71+ cells, and then finally to TER119+CD71− cells [25].
Questions remain, including how to induce stem cells to grow into the precise type of cell needed, then how to persuade those cells to function properly.
The cells were then incubated overnight to allow the cells to re-adhere to the plate.
Assays were normalized first to cells alone and then to a vehicle control.
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CEO of Professional Science Editing for Scientists @ prosciediting.com