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Tsang et al. (2011) examined the response of root epidermal cells to perturbations of the cell wall.
In order to further investigate the role of UNC-34 at epithelial junctions, we took advantage of a hypomorphic mutation affecting the C. elegans α-catenin, HMP-1, which sensitizes cells to perturbations in cadherin function [16].
Cell rheology experiments have indicated the presence of distinct regimes of mechanical response to perturbations at very short (10−3– 1 s), short (1 10 s) and intermediate (10– 10 s) timescales [ 4– 6], while creep and stress relaxation studies have highlighted the relative contributions of elastic and viscous effective responses of cells to perturbations across time [ 7, 8].
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It has been proposed that environmental and mutational robustness may be aspects of the same phenomena: resistance of the cell to perturbation [ 13, 18, 27, 28].
On the surface, transcript profiling using microarrays seems to offer a way of looking at the global response of the cell to perturbation, with a focus on changes in gene expression.
In summary, the selected set of genetic perturbations targeting sphingolipid metabolism translated to a remarkable heterogeneity in lipid states, revealing considerable tolerance of cells to such perturbations and establishing these as an effective method to alter cellular lipid composition and study the functional consequences.
Researchers discovered many small nuances in pluripotency states of stem cells by subjecting the cells to various perturbations and then analyzing each individual cell to observe all the different reactions to developmental cues within a stem cell colony.
10.7554/eLife.04640.008 Figure 2. Response of melanoma cells to systematic perturbations with targeted agents.
Next, we profile proteomic and phenotypic response of cells to the perturbations.
The high-throughput phenotypic and proteomic profiles form a response map of cells to systematic perturbations.
Our experimental conditions do not allow for the ultimate proof of the existence of a proper attractor, i.e. the robustness of the differentiated cells to external perturbations, but the obtained results globally support the idea of attractor.
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Justyna Jupowicz-Kozak
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