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A niche constitutes a number of intrinsic and extrinsic factors such as transcription factors, growth factors, extracellular matrix, neighboring cells, substrate stiffness, and environmental signals.
For loading the cells, substrate was diluted directly in the medium for a final concentration of 1.5 µM and incubated for 60 min at room temperature and then replaced with fresh medium.
In intact cells, substrate delivery is under complex metabolic regulation.
The force will vary with the cell type, the physiological state of the cells, substrate and buffer preparation, etc.
As with isolated mitochondria, the proton current generated by basal respiration supplies ATP synthesis and the proton leak, but in cells substrate supply and ATP demand are both strongly influenced by extramitochondrial processes not necessarily under the control of the experimenter.
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At least three cellular phenotypes have been identified in neuroblastoma cell lines; neuroblastic N-type cells, substrate-adherent S-type cells and intermediate I-type cells [2 5].
Second, we only used one commercial HEp-2 cell substrate.
Thermally cross-linked glucose-containing electrospun gelatin meshes were studied as possible cell substrate materials.
No difference was observed between wildtype and double null neurons on control CHO cell substrate.
These data suggest that integrin endocytosis is regulated by activin induction, cell substrate adhesion, and through interactions with kermit2.
All samples were also tested for anti-nucelar antibodies by immunofluorescence on a HEp-2 cell substrate (ANA).
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