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7-AAD was added at a 1 50 dilution (BD Biosciences, San Jose, CA) and the cells strained through BD FACS tubes (Corning, Union City, CA) before analysis.
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The resulting construct was linearized with SalI and purified prior to injection in ES cells strain 129 by electroporation.
E. coli cells, strain DH5α cells with pUC19 plasmid, were grown in a LB broth at 37°C overnight.
Two kinds of acute cell straining protocols were employed on the epifluorescent microscope-incremental stretches, and stretch-relax trials.
The silo was instrumented with wall pressure cells, free field cells, strain gauges and displacement devices.
An automatic data logger unit had been used in order to record and store data during the test for load cells, strain gauges and the LVDTs.
Also, growth rate is inversely related to copy number across cells strains as well as by altering media composition for a given cell strain [28], [29].
The FSMAD1 construct (described above) was linearized and electroporated into feeder-free E14Tg2A.4 embryonic stem cells (strain 129/Ola, BayGenomics).
Bloodstream cells (strain 328.114) were grown in HMI-9 media containing 10% fetal calf serum and G418 (2.5 µG418−1) [69].
Left images show exponentially growing divICts cells (strains RL5927, 5928), and right images show a sporulating Δ divIB cell (strain RL5929).
Briefly, iNOS oxy containing plasmids (pCWori) were transformed into competent Escherichia coli cells (strain BL21).
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