Sentence examples for cells sphere from inspiring English sources

To analyze the stemness-associated properties of the isolated PROM1/CD133+ cells, sphere forming, stemness gene expression and drug sensitization assays were performed.

Although HLE-sphere cells as well as SK-sphere cells showed significantly increased resistance to several anti-cancer drugs and antioxidant potentials compared to parental cells, sphere cells from HLE cells seem to be cells bearing cancer stem-like properties with incompleteness in morphology, stemness marker, and ABCG2 expressions.

Compared with bulk cultured cells, sphere-derived cells showed more cellular homogeneity in scatter patterns suggesting an element of cell selection (Fig. 1B).

Because each neurosphere was derived from a single cells, spheres were either entirely positive or negative for YFP (Figure 4A).

Second, BKM120 eliminated MCF-7/A02 and CALDOX cells' sphere-forming efficacy (SFE), as well as the ability to produce colonies.

For experiments with adherent cells, spheres were dissociated and plated in N2 medium supplemented with 1% foetal bovine serum (FBS).

DU145 PC stem-like cells (spheres) were isolated and propagated as we have previously published (Rybak et al, 2011).

Under the culture conditions identical to those for neural stem cells, spheres resembling typical neurospheres readily formed on top of the monolayers of both cells.

For splitting cells, spheres were spun down (300 g, 5 minutes, RT) and dissociated with 1 to 2 ml Accumax (Abcys, Paris, France) for 30 minutes at 37°C.

Conventional approaches, including the hanging drop method and suspension culture, are used for cell sphere production.

Single-cell suspensions were plated at clonal density to minimize the effects of cell aggregation in favor of single-cell sphere generation.