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Two platinum electrodes placed in both glass cells solution served as working electrodes whereas saturated calomel electrode (SCE) worked as reference electrode to monitor the potential applied.
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Before adding to the suspension cultured cells, solutions of SWCNTs were sterilized by boiling for 30 min.
The cells solutions were then incubated at 30°C for another 2 hour and were ready for eDNA extraction.
Tissue specimens or single cells solutions were fixed with 7% formaldehyde before analysis.
The cell solution was then extracted.
The cell solution was centrifuged at 250×g x 10 min to obtain a cell pellet.
Perlingeiro and her colleagues then injected the mouse with the muscle-cell solution.
All parasite and cell solution dispensing was performed using the GNF Systems Washer/Dispenser II GNFF).
A 1 1 fibrinogen and cell solution mixture was made by mixing the same volume of the fibrinogen solution and the cell solution, resulting in 1 mg ml−1 fibrinogen and 5,000 cells ml−1 in the mixture.
The cell solution was then filtered and cells were incubated with PI (1 μg/ml) on ice.
This cell solution was filtered and incubated with PI (1 μg/ml) at room temperature for 10 minutes.
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