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In some coelenterates, cells at the vegetal pole do not form an invaginating pocket, but individual cells slide inward, losing connection with other cells of the blastoderm.
Agreement was achieved on the following topics: choice of cells, slide preparation, analysis of micronuclei, toxicity, use of cytochalasin-B, number of doses, and treatment/harvest times [Environ. Mol. Mutagen. 35 (2000) 167].
Tiny cells slide into tiny cells.
In contrast, cells in aggregates with viscoelastic-liquid properties, under comparable compressive force, take much longer to regain their original shape as cells slide past one another and overcome the arising friction [7], [8], [9].
It has been found that the daughter cells slide side-by-side during the first round of division and, after the second round of division, the resulting offspring cells are all arranged in 4-cell arrays.
If rows of cells slide past each other, cells typically engage in T1 transitions.
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So check back when more lawyers in suits pony up to another microphone and we feel more of our brain cells sliding into a deep freeze.
For detecting fusion with host cells, slides were also incubated with a mAb against chicken specific antigen (8F3, Developmental Studies Hybridoma Bank at the University of Iowa).
For detection of CD3 expressing cells, slides were boiled in 10 mM Na-citrate (pH 6.0) for 20 min. Endogenous peroxidase activity was blocked with 0.3% H2O2 in methanol.
For DT-40 cells, slides were also coated with Cell-Tak according to the manufacturer's instructions (BD Biosciences).
Analyses of ANAs were performed by indirect immunofluorescence on human epidermal cell 2 (HEp-2 cells) slides (Immunoconcept, Sacramento, CA, USA) using 1 100 diluted samples.
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