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These adjustments make possible the observation of the effect of each parameter on: (1) cell growth; (2) lipid accumulation; and (3) cells' shape changes.
This coupling process was developed for the first time in this study to observe online and in real-time the most phenomena occurring over the culture (cell growth dynamics, cells' shape changes, cells' fluorescence, and lipid assimilation) according to the culture factors in each of the bioreactors.
These factors could include cytoskeletal rearrangements and expression, calcium fluxes, cell interactions with stroma, matrix, or other cells, shape changes, altered expression of import proteins, or a specific phosphorylation signal on ERK [42].
The cells shape changes during the flow do not appear to be associated with minimization of the surface fluid stresses.
In motile cells, shape changes are strongly related with the modulation of cell adhesions to the substrate via the forces they exert on it.
Using high-resolution live imaging, Bosveld et al. performed a fine and precise quantitative analysis of cellular features and were able to assess the contribution of the cells' shape changes and rearrangements to tissue morphogenesis.
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Actin filaments sustain the cell membrane and their dynamics insure cell shape changes.
Interactions between the actin cytoskeleton and the adherens junction are required for certain cell shape changes.
These data suggest that cell shape changes that accompany EMT are critical for induction of the myofibroblast phenotype.
Fig. 11 Monitoring culture parameter effect on cell shape changes reviled by online flow cytometry and optic microscopy.
We found that apical actomyosin contractions began before cell shape changes in both Caenorhabitis elegans and Drosophila.
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