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An estimated 700-fold enrichment for homologous versus nonhomologous integration events was obtained, such that 70% of cells scoring positive for Thy-1 were derived from gene targeting.
An unique advantage of our approach is that FRET-efficiency can easily be quantified as percentage of cells scoring FRET-positive.
Cell survival in the chips was monitored by time-lapse imaging of cells labeled with the vital dye calcein that leaks out of dying cells, scoring the total number of living cells for each time-point.
The intensity of the cells scoring moderate or higher was judged as positive.
Multinucleate cells scoring positive for tartrate-resistant acid phosphatase (TRAP) were also induced from CD14-positive cells in the synovial fluid from patients with RA [ 17].
The absolute number of multinucleate cells scoring positive for tartrate-resistant acid phosphatase was significantly decreased in the RNODN-treated group.
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After amputation, the fish were incubated in the JNK inhibitor (SP600125) or vehicle and TUNEL-positive cells scored at 18 hpa (Fig. 5b).
The signal pattern was consistent in 100% of interphase and metaphase cells scored.
The analysis of aqueous cells score, flare scores, cell counts, and flare value was conducted with a repeated measures analysis of variance at postoperative visits.
The MN frequency was calculated as MN%= Number of cells containing micronucleus Tota number of cells scored × 100.
To her surprise, those cells scored positive, indicating that they naturally contain the leptin receptor.
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