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A nonspecific toxicity test is surely more useful, at least for a first approach, than specific ones; in addition, the use of yeast cells respects ethical principles (no use of higher animals) and, especially, the toxic effects on these cells result very similar to those on human ones [26].
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The expression of CYR61 is higher in adjacent cells respect to PIN and Meta cell populations (Figure S8).
This discrepancy might be due to a different and fine regulation of EGFR expression in PTC cells respect to normal controlateral thyroid cells.
Glucose starvation for 12 hours resulted in slight increase in the number of double positive Annexin V-PI cells respect normal growing conditions: 1.27 fold for UACC903 cells and 2.35 fold for A375 cells.
The expression of Angiotensinogen (AGT) is higher in adjacent cells compared to PIN, L-PCA and H-PCA whereas JAG1 follows an opposite trend (down regulated in adjacent cells respect to L-PCA, H-PCA and Meta).
On the whole MMP2 and MMP14 expression was higher in the alveolar RH30 cells respect to the embryonal RD.
Microarray analysis revealed a specific transcriptional program induced by HCV in replicon cells respect to both IFN-α-cured and Huh-7 cells.
Interestingly HIF-1α inhibitor determined, after 48 h of incubation in presence of PC3 CM, a significant reduction of WI-38 cells respect to control cells cultured without PC3 CM, suggesting that conditioned fibroblasts developed a novel addiction for glycolysis.
Accordingly, layer IV was always preserved in temporal FCD, where SMI32-positive pyramidal cells respected the borders of layer III and layer V/VI and RORß-positive cells remained within layer IV.
They present reasonable evidence that cells respect a boundary between dorsal trunk and dorsal branch that coincides with a domain of Spalt and Knirps expression, and some evidence that local activation of Notch signaling may be involved.
This effect could be the cause of the higher proliferation index found in these samples (up to 23% cells) respect to that observed in both benign diseases and in situ tumors.
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