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To design an appropriate bioreactor for a particular bioprocess, intensive studies on the biological system, such as cell growth, metabolism, genetic manipulation, and protein or other product expression, are needed to understand the cells' requirement on their physical and chemical environment.
The reduced invasion towards LA compared with AA may be because of the cells' requirement to convert LA into AA and to metabolize AA to induce invasion.
We hypothesize that equilibrium exists between the soluble amount of nonpolymerizable and polymerizable tubulins in MC+ cells and the cells' requirement for microtubules.
To determine whether SAGA subunits outside the DUB module affected the cells' requirement for APC/C function, we tested whether deletion mutants of non-DUB SAGA components could suppress APC/C mutants.
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Moreover, the steady state split ratio between glycolysis and the pentose-phosphate pathway of 57% to 43% [ 7] provides further evidence for the cells' requirements for reduction equivalents.
Gene class testing showed relevance of the tricarboxylic acid (TCA) cycle pathway, confirming cell requirement for energy and reducing power after PEF treatments.
Nonetheless, the secretory cell requirement that we had previously documented for the early ovulation was confirmed when we knocked down Hr39 in adult secretory cells.
Given that a T cell requirement was indicated (Fig. 3), we then further determined which subset of T cells is involved in establishing an inflammatory 4T1 tumor microenvironment within the mammary fat pad.
It is unlikely that the β-cell requirement for eIF2 α phosphorylation is mediated through increased translation of Atf4 mRNA, because Atf4−/− mice do not display β-cell defects [76].
Challenges inhibiting c-Jun expression may be due to its low levels or, alternatively, to a cell requirement for c-Jun expression making c-Jun inhibition toxic to the cell.
The increasing red cell requirement, reported not only in the Morris and colleagues study [ 18] but also in the more recent Xtravent study [ 19], represents a meaningful concern regarding ECCO2R use.
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