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To quantify the β-Gal positive cells in each culture condition, we counted the number of blue cells and calculated the % of β-Gal positive cells present in each condition.
The number of cells present in each sample was determined separately using a Bürker chamber.
The gates were set to include the live (PI-) as well as the dead (PI+) cells present in each sample.
If stem cells only divide once per day, there should be 4.1 stem cells present in each inter-villus pocket; if stem cells divide twice per day, there will only be 2.0 stem cells required in each inter-villus pocket.
Cycle numbers of the logarithmic linear phase were plotted against the logarithm of the concentration of template DNA to evaluate the number of yeast cells present in each tissue sample homogenate.
A full dose of genistein (5 µM) combined with a full dose of E2 (1 µM) or a full dose of ICI 182,780 (1 µM) lead to a result similar to that of genistein treatment alone with no statistically different number of apoptotic cells present in each fish (Compare Figure 3D with 3E and 3F).
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We counted the average number of cells present in a cluster for each GFP clone after five days of growth.
tumour implantation by monitoring the in vitro incorporation of tritiated thymidine (3H-TdR) into lymph node cells (LNC) undergoing stimulation in vivo and concurrently determining the total numbers of the lymphoid cells present in these organs at each of the time intervals.
At the end of culture, the cells present in 20 randomly chosen microscopic fields (each 900 μm, containing 50 70 cells) of anther loculi were counted in DAPI-stained median longitudinal sections from ten randomly chosen anthers per treatment and genotype using the Zeiss Axiolab epifluorescence microscope as described above.
Such cells, however, can be attacked by killer cells present in the blood and lymphoid tissues.
They involved Isis cells present in different European countries, including Belgium and Germany, as the early results of the ongoing investigation indicate.
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