After each cell focus had grown to a few hundred cells, portions of each focus were carefully removed using a micropipette tip and transferred into new T-flasks.
"Non-cell portion of the pack is disproportionately high, but we can get it done now instead of February," he wrote.
In the experiments presented in this paper, the deposition destination was chosen to be the cytoplasm center, where cytoplasm is defined as the combination of the yolk and the cell portion of a zebrafish embryo.
To test whether the sensor kinase and carbohydrate binding proteins could be exported from the cell, portions of the genes encoding the N-terminal segments of LevQ (up to Asp202), LevT (up to Asp72) or LevS (up to Ile253) were fused to the ΔSPNuc sequence (Figure S4), which encodes a nuclease derived from Staphylococcus aureus that lacks its export signal [15].
By IHC, XIAP expression was found to be restricted to tubular epithelial and several glomerular cells, and Smac expression was detected in tubular epithelial cells, Bowman capsule cells, a portion of glomerular cells, and fibroblasts in normal kidney.
After co-transfection with two plasmids encoding the respective chimeric receptors in IL-3 dependent murine pro-B Ba/F3 cells, a portion of the cells survived under antigen dependent stimulation without IL-3.
Similar to Id3−/− DN TCRγδ+ cells, a portion of Id3−/− CD4 and CD8 γδ T cells had these activation markers, although the CD4 cells had lower levels of CD122 and only a small portion expressed NK1.1 (Fig. 2B).
Flow cytometry analysis confirmed that in MDA-MB-231 cells, some portion of CD74 was localised on the cell surface.
As in living cells, a portion of the energy-containing material (fuel) not utilized in the engine is exhausted in the form of carbon monoxide, carbon dioxide, and other by-products of combustion.
In cells a portion of cytochrome c (cyt c) (15 20%) is tightly bound to cardiolipin (CL), one of the phospholipids constituting the mitochondrial membrane.
