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Confidence limits on the phase were calculated as: (4) For some cells, phase was linearly related to frequency, indicating a fixed time delay.
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The stem cell phase is chronologically the longest because developing and differentiated cells survive for relatively short periods.
Classification of cell phases was based on the fluorescence intensity of DNA dye.
Cell phases were estimated as a percentage of a total of 10 000 events.
Distribution of cells in distinct cell cycle phase was determined using ModFIT cell cycle analysis software.
The population of cells in each cell-cycle phase was determined by CellQuest Pro Software (BD Bioscience, USA) [ 5].
The cell sorting and cell culture phase was repeated three times.
Distribution of cells in the different cell cycle phase was determined with ModFit LT 3.2.1 or FlowJo 8.6 software.
The cell-cycle phase was assayed by flow cytometry after PI staining of the fixed cells.
The cell growth phase was determined using colorimetric method.
Cell cycle phase was detected using a flow cytometer (FACS420, BD Biosciences, San Jose, CA).
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