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Increased Bmi1 expression was also observed in HRA-19 cells on type I collagen.
The growth of LM8 cells on type I collagen plaques was monitored under an inverted contrast microscope.
This makes it possible to more accurately measure the response of cells on Type 1 collagen than is possible with collagen gels.
The TBSs were derived from in vitro produced bovine blastocysts which were found to form a monolayer sheet of polygonal cells on type I collagen coated flasks.
In fact, it has been recently shown that attempts to grow MiaPaCa-2 cells on type I collagen substrates resulted in apoptosis (Vaquero et al, 2003).
Endocrine lineage commitment was inhibited by the growth of cells on type I collagen, suggesting that type I collagen promotes self-renewal, maintaining colorectal carcinoma cells in an undifferentiated progenitor state.
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To discern the contribution of collagen to the regulation of cellular β-catenin levels, we have cultured DD and PF cells on type-1 collagen-coated trays in the absence of three-dimensional contraction with or without exogenous addition of TGF-β1.
The hCMEC/D3 cells form a contact-inhibited monolayer of elongated cells on collagen type I or type IV.
Paxillin staining revealed that control cells, plated on type 1 collagen, exhibited few focal adhesion structures.
After differentiation the confluent cells cultivated on type I collagen and PEMs were amplified and separated in two batches.
However, it dramatically increased the percentage of adherent cells on the type I collagen matrix.
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