Sentence examples for cells lysis buffer from inspiring English sources

CelLytic B Cell Lysis Reagent (SIGMA B7435) was used for E. coli cells lysis buffer.

The cells were incubated with red blood cells lysis buffer and washed using William's E medium containing chicken serum to eliminate the red blood cells.

After adding red blood cells lysis buffer and 1X PBS washing [ 41], data were acquired and analyzed using a FACSCanto II flow cytometer and analyzed using FACSDiva (BD Biosciences) software.

For adherent cells, lysis buffer [50 mM Tris/HCl (pH 7.5), 1 mM EGTA, 1 mM EDTA, 1% (w/v) sodium orthovandate, 10 mM sodium glycerophosphate, 50 mM NaF, 5 mM sodium pyrophosphate, 270 mM sucrose, 1 mM benzamide, 2 mM PMSF and 1% (w/v) Triton X-100] was added directly to the culture dish before scraping.

Cells lysis buffer contained 50 mM Tris/HCl, pH 7.4, 5 mM EDTA, 150 mM NaCl, 50 mM NaF and 1% Triton X-100, supplemented with 2 mg/ml aprotinin, 1 mM pepstatin, 1 ng/ml L-leupeptin, 1 mM PMSF and 1 mM Na3VO4.

The mammalian cells lysis buffer contained 50 mM Tris/HCl (pH 7.5), 0.15 M NaCl, 1 mM EGTA, 1 mM EDTA, 1 mM Na3VO4, 50 mM NaF, 5 mM Na4P2O7, 0.27 M sucrose, 1% (w/v) Nonidet P40, 1 mM benzamidine, 0.1 mM PMSF, 0.1% 2-mercaptoethanol and Roche protease inhibitor mix (1 tablet in 50 ml).

The cells were lysed in cell lysis buffer containing PMSF for 30 min at 4°C.

Cell lysis buffer and polymerase chain reaction (PCR) kits were purchased from Tiangen, China.

The 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR) and cell lysis buffer were from Beyotime (Shanghai, China).

Cells were washed with 2 mL PBS, lysed with 150 μL cell lysis buffer on ice for 2 min.

1 mL cell lysis buffer and 1 mL acid-washed glass beads (D = 0.5 mm) were added.