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As was observed in the presented case, chordoma tumour cells label with the epithelial marker cytokeratin and S-100, a protein derived from the neural crest and chondrocytes among others.
After culture overnight on fibronectin, 50 80% of trophoblast cells label HLA-G+ by flow cytometry with the mAb G233 2. Conformationally dependant mAb specific for HLA-G were G233 [ 2] from our laboratory, 87G 60] from Dr. D. Geraghty, and MEM-G/11 61] from AbCam.
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(A2) MGcells celabeledeled with QDs.
(B2) MGcells celabeledeled with CC49-QDs.
(D2) MGcells celabeledeled with fluorescent secondary antibody.
(E2) GES-1 cells labeled with QDs.
Orange/red KB cells labeled with folic acid-coated QD.
(F2) GES-1 cells labeled with CC49-QDs.
(H2) GES-1 cells labeled with fluorescent secondary antibody.
However, only the cells labeled with AVEC attracted the anti-HBsAg antibody onto the ovarian cancer cells (d), but the cells labeled with trastuzumab did not (b).
The counts from the cells labeled with therapeutic antibodies and AVEC were statistically significantly higher than the cells labeled with the relevant isotype antibodies.
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