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This non-invasive approach is based on the level of expression of potential biomarkers in cumulus cells (indicators of successful pregnancy) to assess the potential and quality of the embryo.
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Apoptosis was measured using flow cytometry for accumulation of sub-G1 phase cells (indicator of apoptosis) and Annexin V/PI staining.
These indicators are mainly based on the troubled-cell indicators for discontinuous Galerkin (DG) method which are listed in the paper by Qiu and Shu (J.
Qiu, C.-W. Shu, A comparison of troubled-cell indicators for Runge Kutta discontinuous Galerkin mehtods using weighted essentially non-osillatory limiters, SIAM J. Sci.
Qiu, C.-W. Shu, A comparison of troubled-cell indicators for Runge Kutta discontinuous Galerkin methods using weighted essentially non-oscillatory limiters, SIAM Journal of Scientific Computing 27 (2005) 995 1013).
The idea is to first identify "troubled cells" by different troubled-cell indicators, namely those cells where limiting might be needed and discontinuities might appear, then adopt an adaptive approach in these cells.
In this paper, we systematically investigate adaptive Runge Kutta discontinuous Galerkin (RKDG) methods for hyperbolic conservation laws with different indicators which were based on the troubled cell indicators studied by Qiu and Shu [J.
We studied the feasibility of using human fetal skin cells for allogeneic transplantation and showed that fetal keratinocytes have faster expansion times, longer telomeres, lower immunogenicity indicators, and greater clonogenicity with more stem cell indicators than adult keratinocytes.
Similarly, informative priors on cell indicators can help to rule out some sporadic outliers.
The second line of evidence was obtained with a series of fluorescent cell indicators (of pH and Ca2+) conjugated to an acetoxymethylester (AM) [ 28].
Extensive one- and two-dimensional simulations on the hyperbolic systems of Euler equations indicate the good performance of this new troubled-cell indicator coupled with a simple minmod-type TVD limiter for the Runge Kutta DG (RKDG) methods.
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