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CTCs were sorted as unfixed cells in solution.
It was then placed in a bioreactor — a shoebox-size container holding the stem cells in solution — and rotated like a rotisserie chicken to allow the cells to soak in.
The carbohydrate-functionalized CdS nanocomposite showed high ECL sensitivity and good stability, and could be used for competitive recognition to concanavalin A with the target cells in solution, which led to a change of ECL intensity due to the resistance of concanavalin A. The change depended on both the cell number and the expression level of cell surface carbohydrate.
CV results confirm that the anti-EpCAM/LC-SPDP/Au based biosensor could detect MCF-7 cells in the range of 1×105 1×108 with correlation coefficient of 0.999 and detection limit of 1×105 cells ml−1 i.e. 100 cells in solution used for incubation (1 μl).
Conversely, when the concentration of cells in solution A was increased from 30 to 60%%, the number of entrapped cells remained constant.
As a postdoc, he joined Garry Nolan's laboratory at Stanford University, in large part because it was the first lab to try out a new device called a "mass cytometer," or CyTOF, which married time-of-flight mass spectrometry with single-cell analysis of cells in solution.
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Classically, L-forms were isolated by cultivating cells in solutions containing high levels of chemicals that inhibit the synthesis of peptidoglycan, and lytic enzymes that break down cell walls (Allan, 1991).
From 1895 to 1899, Overton carried out a series of experiments in which he immersed cells in solutions of over 500 different substances at the same concentration in order to study their permeability with different molecules [ 66- 68].
The calibration curve for the cilium-targeted ratiometric sensor (mSSTR3-mCherry-GCaMP6s) was obtained by incubating adenovirus-transduced ependymal cells in solutions with free [Ca2+] ranging from 50 nM to 10 μM.
Non-displaying yeast cells (1 × 105) were added to each well and mixed to induced cells to ensure (i) proper cell pelleting and (ii) an excess of soluble CXC chemokine target over total number of yeast-displayed protein binders (5 × 104 copies of proteins per yeast cell) in solution.
Equal amounts of proteins from SIRT3-OE and the control cells were in-solution digested and labeled with TMT reagents.
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