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That is because researchers have figured out how to separate the red blood cells in a sample into groups by age.
Conventional RNA-Seq generates an average summary of mRNA abundance across all of the cells in a sample.
In-flow counting enables a rapid assessment of the cancer-associated large circulating cells in a sample within minutes to determine whether standard downstream assays such as cytological and cytogenetic analyses that are more time consuming and costly are warranted.
In particular, Mass Cytometry Time-of-Flight (CyTOF) is a high-throughput single-cell technology that enables the simultaneous measurements of multiple ([Formula: see text]40) intracellular and surface markers at the level of single cells for hundreds of thousands of cells in a sample.
In this condition most of the red cells in a sample of fresh blood look normally shaped discoidal until deprived of oxygen, when the characteristic sickle- or crescent-shaped forms with threadlike extremities appear.
Advances in medical science have shown importance of performing analysis of heterogeneous cells in a sample, e.g. tumor, circulating cancer cells (CTC) and blood, etc. [1 4].
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Solid-state micropores can characterize each cell in a sample providing interesting information about cellular populations.
A key advantage of visual plate counting is its sensitivity for detecting growing cells; a visible colony will develop even when there is only a single culturable cell in a sample.
The DNA fragmentation index (DFI) value was calculated for each sperm cell in a sample, and the resulting DFI frequency profile for the entire sperm population was obtained.
Although the term 'nonculturable' is quite commonly used to describe not-immediately-culturable cells it is best avoided, as we cannot try every possible combination of incubation conditions that might serve to resuscitate a cell in a sample.
This technique allows measurement of fluorescence in up to 107 individual cells in a single sample, with multiple samples run for a single experiment.
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