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We define network cells for lifetime purposes.
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By shutting down the expression of most EBV-encoded antigens, EBV-infected memory B cells become invisible to the immune system and circulate permanently in the blood in low but stable numbers (∼1/10 B cells) for the lifetime of the host (Latency-0/-I or true latency programme; Khan et al., 1996).
Though select areas of the cells were chosen for lifetime analysis, the area selected was representative of the dye distribution within the cytoplasm, resulting in a homogeneous monoexponential fluorescence decay.
Calculated CFP lifetimes were referenced to Rhodamine-G6 which was set at 4.11 nsec, and internally calibrated using cocultured CFP containing MelJuso cells for which the lifetime was set to 2.7 nsec, as we applied before [ 36].
Worth considering is that environmental induced epigenetic mutations in developing germ cells may have consequences for lifetime fertility as there is likely to be plasticity in histone modifications.
The biological processes of cell death and cell cycle were enriched both for lifetime and progression-free survival.
For lifetime experiments, cells were kept in HBSS to avoid background fluorescence.
These processes included: aging, morphogenesis, cell cycle and proliferation, and death for lifetime survival; morphogenesis for overall survival; and cell cycle, death and recognition, death, response to biotic and abiotic stimuli, programmed cell death, and apoptosis for progression-free survival.
It is important for stem cells to maintain lifetime self-renewal and proliferation.
John Baldessari and Yoko Ono received Golden Lions for lifetime achievement.
7. Credit for Lifetime Learners.
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