Sentence examples for cells for library from inspiring English sources
The plasmid was cotransfected with a helper plasmid into HEK293 cells for library production and preparation.
Once we confirmed a robust immune response towards SEB, we isolated RNA from the llama's white blood cells for library construction.
The libraries were then transformed into ElectroMAX™ DH10B cells (Invitrogen) for Library 2 or ElectroTen-Blue™ cells (Stratagene) for Libraries 3 to 6.
It is not clear whether selection of this clone is an artifact of the experiment, or the result of an aberrant genome rearrangement in the tumor cells used for library construction.
Genomic DNA isolated from the undifferentiated (E14-d0) and differentiating (E14-d6) states of mES cells were used for library construction.
In MC and MN media the cultures did not reach an O.D. of 1.2 and RNA isolated from more than one flask of cells was used for library creation.
After 48 h of Puromycin selection and another 2 days of culture, mCherry positive (as a marker for library infected cells) cells displaying reduced GFP levels were sorted using a FACSAria cell sorter.
We selected 4 cell lines, HeLa cervical cancer cells, IMR32 neuroblastoma cells, MCF7 breast cancer cells and T47D breast cancer cells, for generation of libraries.
After 24 h, cells were recovered by centrifugation and plated in fresh media containing puromycin (1 µg/mL) to select for library transduced cells.
The first approach simply repeated the RED assay measuring the equilibrium position in the presence of different amounts of either PS-rich vesicles (POPC Cholesterol POPS, 65:25:10) or PC vesicles (POPC Cholesterol, 25:75; mimics of healthy cells) such that association constants for library candidates 11k and 11o could be determined.
Moreover, since the absence of Xrn1 affects the overall mRNA content (Sun et al., 2013), an aliquot of a S. pombe culture was added to the cell pellet before RNA extraction for library preparation to provide an independent internal control for normalization.
