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YC company Cytera builds machines that automate the growing of human and animal cells for biotech.
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The cells were harvested at OD600 4.0 and washed twice with sterile water and resuspended in 100 mmol L−1 Tris HCl (pH 7.5) with a proteinase inhibitor cocktail (for fungal/yeast cells; Sangon Biotech Co., Ltd., Shanghai, China).
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In San Diego biotech Fate Therapeutics is collaborating with biotech toolmaker Stemgent to form Catalyst, a venture to make IPS cells for other firms that join them in a consortium.
Apoptosis was evaluated by Annexin V-FITC/PI staining reagent (Yeasen Biotech, Shanghai, China) in ganetespib-treated or siRNA-transfected cells for 72 h.
CD4 T-cells cells for cytokine and proliferative assays were isolated from PBMNC by negative selection with immunomagnetic beads (Miltenyi Biotech, Auburn, CA).
PrimeGen, a small biotech company based in Irvine, CA, says that it has solved one of the major hurdles in using reprogrammed stem cells for human therapies.
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