Sentence examples for cells fixation from inspiring English sources

In contrast, Bodipy and plasma lipids did not form into lipid bodies in PBMC, thus, escaped PBMC following cells fixation and permeabilization.

Firstly, intracellular staining requires cells fixation and permeabilization and these procedures alter the light-scatter characteristics of the cells, increases autofluorescence and reduces the reactivity of the other fluorochrome-labeled antibodies included in the assay [37].

For IFA of LS in infected HepG2 cells, fixation was either 4% paraformaldehyde (PFA) + 0.1% triton or acetone, and incubation was with anti-LISP1 antibodies and Alexa 647-conjugated secondary antibody.

The harvesting of cells, fixation and preparation of metaphase spreads for chromosome banding analysis and FISH were carried out from cytogenetic pellets of cultured cell lines according to protocols described in Courtay-Cahen et al (2000).

While after fixation with conventional glutaraldehyde a homogeneous tubule epithelium is seen consisting only of bright cells, fixation by glutaraldehyde including tannic acid illustrates a heterogeneously composed epithelium consisting of bright and dark cells.

5, 8 Fluorescent labeling of plasma membrane for TIRFM was obtained by incubating cells with 15  µg/mL 4- 4- dihexadecylamino)styryl)- N-methylpyridinium iodide (DiA; Molecular Probes, Invitrogen) for 4- 4- dihexadecylaminoe experimentstyrylls fixation was obtained by 10 min incubation with 3.7% paraformaldehyde (Sigma).

Understanding the detailed process of cell fixation in various states from living cells to completely fixed cells provides an opportunity to optimize cell fixation protocols and to gain useful knowledge about the living cell fixation process.

Also cell fixation results in a decrease of their entrapment due to an increase in cell rigidity by the fixation process.

4%, which is consistent with the empirical concentration of cell fixation optimized in biological protocols.

Then formaldehyde (Sigma, USA) in 3.7% PBS was used for cell fixation followed by further washing.

Furthermore, cell fixation provides an effective approach for immunostaining by allowing the antibodies to access intracellular structures [5].