Exact(8)
Harvesting of cells, extraction and analysis were performed according to the recommendations of the manufacturer of the MIDI identification system (Microbial Identification System).
The step-by-step protocol includes isolation of these cells, extraction of nuclei, fixation, restriction digestion, ligation, and whole genome amplification.
Methods for vascular reactivity performed in mice [26], [27] and endothelial cells extraction and culture were set up as previously described [19].
CX performed the BM cells extraction, transplantation and immunochemistry assessments and participated in the sequence alignment.
Four- to five-week-old congenic male DL mice were selected for BM cells extraction.
For tissues and cells, extraction of lipids was performed using Bligh and Dyer method.
Similar(52)
Protein extracts were obtained using Cell Extraction Buffer buffer (BioSource International, CA, USA) plus protease inhibitor cocktail (Sigma).
Proteins were extracted using a Whole Cell Extraction Kit (EMD Millipore Corporation, Billerica, MA, USA), according to the instructions.
Nuclear extracts were prepared as previously described using a cell extraction kit (Active Motif, Carlsbad, CA, USA).
Protein extracts were normalized to wet weight in an appropriate volume of cell extraction buffer.
He described pepper spray and forcible cell extraction.
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