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Since nrGO was sterile, it was used for cells experiments directly.
There was no significant effect of the source of the donor nuclei (ear skin fibroblast or cumulus cells), the age of the animal (3 months or 4 years) from which the donor cells were derived, serum deprivation of the donor cells, or the use of fresh or frozen-thawed donor cells (experiments 2 5).
Resting cells experiments were performed in triplicate.
For each experiment we analyzed at least 10 representative cells; experiments were routinely repeated twice.
Parallel to experiments with C4-2LucBAD cexperimentsiments were conducted with C4-2Luc cells in which endogenous BAD expression was inhibited by the shRNA approach.
To determine if Rituximab is acting directly on T cells, experiments were performed to examine the expression of hCD20 on B and T cells.
Similar(20)
And his stem-cell experiments contribute to his pioneerlike mystique.
Mr. Bush struck a careful compromise that he said would encourage potentially lifesaving research but discourage experiments on human embryos, which are destroyed in stem cell experiments.
And Mr. Bush singled out research cloning for special criticism when he announced last week that the government would pay for certain limited stem cell experiments.
A group of American and Canadian biologists is debating whether to recommend stem cell experiments that would involve creating a human-mouse hybrid.
Mr. Bush opposes cloning and has restricted federal financing for human embryonic stem cell experiments, so the White House has searched for scientists who support those positions.
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