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Reproducible results were obtained by using another independent clone, SK-ES-DKK1#2 cells (experiment #2 in Table. 1).
Although PEBP (CBFb) was only marginally enriched (p∼0.1) in this list, it appeared among the ten top TFBSs exhibiting the lowest p-values both in the lists derived by DEMON from consensus data sets (ranked 2nd and 3rd) and from the hIPCs vs. PANC-1 cells experiment data set (ranked 6th).
This occurred for both blood cells (experiment 1 only; Figure 2A) and gonadal cells (both experiments; Figure 2B).
Moreover, we performed a fine specificity analysis of the T-cell response against CII as well as CD4/CD8 phenotyping of the CII-responsive T cells (Experiment II).
Particularly interesting were the cluster 4 and 7 genes, which were regulated by STATc and this regulation also dominated the transciptional output of wt cells (experiment I).
We used the comet assay to determine the level of DNA damage (DNA strand breaks) in gonadal cells (both experiments) and/or blood cells (experiment 1 only) of all fish.
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Since nrGO was sterile, it was used for cells experiments directly.
Resting cells experiments were performed in triplicate.
For each experiment we analyzed at least 10 representative cells; experiments were routinely repeated twice.
This is not the first stem cell experiment in people, however.
For the stem cell experiment, Dr. Hodgen and his colleagues selected 12 women who had already volunteered as egg donors and two men who had volunteered as sperm donors.
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