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This presumably reflects the "oncogenic" transformation of the cancer cell lines relative to normal cells, events that reflect the multiple mutations that characterize cancer cells.
To identify CD4+ and CD8+ T cells, events from R1 were analyzed in a plot of CD3-FITC vs CD4-PerCP or CD8-PerCP respectively (R2).
Following these adjustments, the number of TUNEL positive stained cells ("events") was automatically counted and the total hepatic area was recorded.
We noted at the 72 hr time point p.i. (left panel Figure 1A) a small number of "cells" (events) which lay within the CD8+ T cell flow cytometry gate and which already had diminished CSFE intensity.
Experimentally and clinically, CNS damage is followed by an acute and a prolonged inflammatory response characterized by the production of inflammatory cytokines, infiltration of leukocytes and monocytes in the injured area, as well as the activation of resident glial cells, events that may contribute to secondary CNS injury [34] [36].
The cells were then incubated with 100 µg/ml RNase Boehringer Mannheimm) for 15 min, followed by 40 min of incubation in freshly prepared PI (5 µg/ml).At least 10,000 cells events were recorded for each sample using a FACSCalibur (BD Biosciences) and analyzed using the CellQuest program.
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A cluster schedules its own events, which run at the same frequency of cell events.
RAW cell events were analyzed by an external RTX (anti-human) versus total RTX plot.
The critical endothelial cell events contributing to angiogenesis that are inhibited by radiation are migration and sprouting.
A large number of flow cell events (typically 2×105 cells) were pooled to represent each experimental set in order to minimize noise.
As reported above, we have shown that MPsShh+ are able to differentially regulate cell events leading to in vitro angiogenesis [8].
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