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Tracheary element differentiation was highest, ranging from 20 to 40% of the cells, directly after the initiation of suspension cultures, and declined with the duration of culture to 5 to 10% of the cells (duration of culture unspecified).
Total interaction assays and LDH toxicity assays (data not shown) were used to optimize several factors, including degree of total interaction between bacteria and cells, duration of interaction, and the amount of host cell damage incurred by HIGK during the co-culture.
The ictal events were also significantly longer-lasting in Rac1N than in control cells (duration of ictal-like activity: 53.6 ± 8.5 vs. 22.3 ± 1.8 s) (Fig. 6 C).
Logistic regression was used to analyse the effects of factors (baseline FEV1 and sputum inflammatory cells, duration and type of exposure) that may potentially influence FEV1 decline.
For the control case, as mentioned in the previous section, we suppose that in the course of ring migration which average (over 7 cells) duration is ∼110 min, C increases linearly from 2.1 to 3.3 while L increases linearly from 1.6 to 2.8.
This apparent discrepancy with the current study might be due to the different animal models used (mouse vs. rat), the different cell lines used to induce tumor formation (murine C-26 adenocarcinoma vs. MH7777 hepatoma cells), duration of tumor development, and/or the level of physical activity of the animals.
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The mutational variants can further affect centrosome size, movement, the number per cell and cell cycle duration as well as cell size and embryo size.
In this way, upward and downward causation regulates both, the cell cycle duration and cell size homeostasis.
Sponges are known to have high proliferation activity, a short cell cycle duration and cell shedding that is predominantly confined to the choanocytes [e.g. [ 54]].
One of the processes, which have been studied for at least several decades, is regulation of cell size and cell cycle duration.
The AMT technique was optimized by altering the concentration of acetosyringone, the ratio and concentration of A. tumefaciens and A. aculeatus cells, the duration of co-cultivation, and the status of A. aculeatus cells when using conidia, protoplasts, or germlings.
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