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(A) Gating strategy from one donor is illustrated; cells debris, doublets and dead cells were excluded from analysis.
Antigen can be loaded as exogenous cells, debris, protein or peptides and by endogenous production through genetic modification.
The remaining non-burst cells and cells debris were centrifuged at 3000 rpm for 10 min.
As the device thoroughly buffed my skin, it would simultaneously vacuum away everything dulling my complexion (think oil, dead skin cells, debris) and flood my freshly-cleansed skin with serums.
Phagocytosis of marrow cells was defined as the presence of intact cells (as opposed to cells debris), such as red cells, erythroblasts, platelets, or white cells, in the cytoplasm of macrophages.
The harvested recombinant E. coli BL21 DE3 pLysS AcCR) cells were suspended in 50 mM citrate phosphate buffer (pH 6.5) at the concentration of 50 mg/mL, ultrasonicated for 3 × 18 min, and then the cells debris was removed by centrifugation at 12,000 rpm at 4 °C for 20 min.
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Cell debris was cleared by centrifugation.
However, microglia cells showed endocytic vesicles containing only bacterial cell debris.
Cell suspensions were homogenized by 20 strokes of a dounce homogenizer and cell debris was pelleted.
Nuclei and cell debris were removed by centrifugation at 13,000 × g for 5 min at 4 °C.
Plates were washed lightly with sterile 1X PBS to remove cell debris.
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