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(3) We compared the average gene expression of selected CD41a+ cells (days 5 12) against equivalent-day cells from concurrent, isogenic G cell cultures (n = 2).
Cell samples were flash frozen in liquid nitrogen at day 0 for CD34+ cells; days 1, 2, 3, 4, 5, 7, 9, and 12 for Mk cell cultures; and days 1, 2, 3, 4, 5, 7, 9, and 11 for G cell cultures.
Figure 2 Hospital mortality (%, 95% confidence interval) according to maximum age of red blood cells (days) from Pettila et al.[15]with permission.
Upon the appearance of a cytopathic effect (CPE) or after ten (or thirteen in the case of Vero cells) days of culture, the cells were spotted onto microscope slides.
Interestingly, levels of B. hermsii in the blood of mice which received wild-type B-1P cells were significantly higher than in the mice that received IL-10−/− B-1P cells (days 2 to 4, Fig. 7D).
GFP-P19Cl6 cell culture was digested to single-cell suspension with Accutase (Cat. No. AT104, Innovative Cell Technologies, Inc., San Diego, CA, www.innovativecelltech.com) for adhered cells (days 6 and 14) or Accumax (Cat. No. AM105, Innovative Cell Technologies , Inc. for suspended EBs (day 5 of differentiation).
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We used flow cytometry to allow us to gate specifically on CD71+ cells (day 7) or CD235a+ cells (day 14).
Fetal distal lung epithelial cells (day 19) were isolated as described previously [22].
Distal fetal lung epithelial cells (day 19 of gestation) were isolated as previously described [24].
Total RNA was extracted from day 0 undifferentiated cells, day 1 ectodermal cells, day 3 FACS-purified Sox1-GFP+ and Sox1-GFP++ NPs, and day 8 neuroepithelial rosettes, using High Pure RNA Isolation kit (Roche Diagnostics).
A GeneChip Exon Array was performed using total RNAs purified from undifferentiated P19 cells (Day 0), neuronal differentiated cells (Day 7), and cells from the early glial stage (Day 10).
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