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Moreover, this approach preserves the cells contour, an important feature because of the performance of bioreactors and other chemical processes are greatly influenced by their morphological character.
Microscopy of the TKO MEFs revealed them to be abnormally large compared to WT cells (contour in Figure 1C).
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Depending on how far senescent degeneration has progressed, fragmentation of the microglial cytoplasm (cytorrhexis) may be partial or it may involve the entire cytoplasm leaving multiple fragments that in some cases still delineate the cells' contours, or in the most advanced case of disintegration are widely scattered about.
The cell contour at 60 min is outlined in solid white, whereas the initial cell contour (0 min) is outlined with dashed white.
The efficiencies of 6-cell tile shaped aerospike nozzle and 1-cell linear aerospike nozzle at design altitude approach to 100%, and that of 6-cell aerospike nozzle with round- to- rectangle primary nozzles in the same condition is about 95% due to the imperfect cell contour and manufacturing defects.
Since the concept of MA is well translated into microfluidic, there are many previous works reported on studying of kinetic of cell shape deformation during entry, cell contour tracking [58] and nucleus deformation [59].
First, if the function of cell contour becomes a multivalued function, we employed the value furthest from the centroid.
Thus, only puncta localized on or near the plasma membrane, which was visualized as a cell contour, were counted.
This result clearly highlights the importance of precise cell contour identification to the analyses of cell-to-cell variations within a population of adherent cells.
Therefore, it is difficult to identify the cell contour of such adherent cells and to obtain quantitative measurements that are comparable to those from flow cytometric analysis.
The development of customized algorithms allowed the detection of individual cell contour and the use of cell morphology endpoints for toxicity reporting (figure 3).
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