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For solid (precipitated minerals and/or cells) collection, the supernatant was removed after centrifuging (8,000 × g, 20 minutes) and then the solids were washed 5 times with ethanol and repeated centrifuging to remove residual salts.
Physicochemical parameters analyses, cells collection, DNA extraction, 16S rRNA gene Illumina sequencing and data processing were carried out as described previously (Xiao et al. 2015) and also see a detailed description in supplementary materials.
Following cells collection, total RNA was isolated with the PicoPure RNA isolation kit (Arcturus; CA) according to the manufacturer's instructions.
Viral titers of the oropharyngeal and cloacal/fecal swab samples were calculated as 50% tissue culture infectious dose (TCID50) per ml swab sample on Madin-Darby canine kidney (MDCK) cells (Collection of cell lines in veterinary medicine, FLI Island of Riems, RIE1061).
fIsolated from a bluejay (Cyanocitta cristata) and passaged once in Vero cells (collection date: 06/14/02).
Buccal cells collection vials were returned to the National Cancer Institute (NCI) repository via a postage-paid, padded envelope.
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There were no purged or CD34-selected stem cells collections.
We collected the HeLa S3 cells by using standard cultured cell collection procedures.
The stem cell collection, instead of taking three days, they probably had enough in one day.
Most donations - 90 per cent - now take place via peripheral blood stem cell collection, which is done at an outpatient appointment and is similar to donating blood.
Like Dr. Fisher, their treatment could start with the oral drug Revlimid or the intravenous treatment, Velcade, followed by stem cell collection.
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