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As with uninfected cells, cleavage of eIF4G strongly inhibits translation both of cap-Luc-poly(A) and C+Luc mRNAs whereas protein synthesis directed by IRES-Luc mRNA was stimulated.
In human mitochondrial extracts prepared from different types of cultured cells, cleavage by mtRNase P seems to generally occur first.
In cells, cleavage of PrPc by members of the desintegrin and metalloproteinase family of proteases dissociates the disordered N-terminus of PrPc from its membrane-bound C-terminus.
Although the hnRNP K cleavage product could be detected when the reaction was performed in cytoplasmic extract from ctrl siRNA transfected cells, cleavage was strongly reduced in extract prepared from Caspase-3 knockdown cells.
32 Following HCV infection of HDFR-expressing cells, cleavage of IPS-1 by the viral NS3-4A protease 2 leads to migration of the fluorescent reporter from mitochondria to the nucleus, enabling visualization of HCV-infected cells.
Pharmacologic inhibition of caspases using Z-VAD-FMK inhibited [Gem+Clo+Ed]-mediated cell death as suggested by the low percentage of Annexin V-positive cells, cleavage of PARP1 and caspase 8, and phosphorylation of histone 2AX.
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Although not directly related to the ANLSH, there is evidence that monocarboxylates can act as rich energy sources for cells: cleavage-stage embryos, for example, initially require pyruvate but they switch to glucose as the preferred energy source as the embryo develops into a morula [ 13].
The constantly changing arrays of actin filaments help move the cell and mediate specific activities within it, such as cell cleavage during mitosis.
This indicates that the basic cell architectures of early embryos are largely established by the autonomous activities of the mitotic apparatus, without much dependence on the cell cleavage machinery.
Three of the mutations (acytokinesis mutations: ackkt5, ackkt62 and ackkt119) caused absence of cell cleavage in the embryos derived from homozygous females regardless of the paternal genotype, without affecting nuclear divisions.
"These sorts of [developmental] dynamics are not only seen in snails, they are involved in all cell cleavage".
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