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The experiment was based on a previously described method [ 27] adapted to cell-based assay.
Notably, this assay provided greater sensitivity in detecting seeding by polyQ than the cell-based assay to monitor visible aggregates (Figure 2b).
(a) Cell-based assay of canonical Wnt pathway activities through a TCF/LEF luciferase reporter system composed of the TOPflash and control FOPflash constructs.
MSH2 promotes CAG repeat contractions together with the NER factor, Xeroderma Pigmentosum, Complementation Group A (XPA 15, in a human cell-based assay.
Bao, Y. et al. A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription.
Regardless of the technology (RNAi, CRISPR, over-expression, etc)., a good cell-based assay is the best foundation for a cell-based screen.
We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster.
Efficacy for the inhibition of Dyrk1A mediated tau phosphorylation was demonstrated in a cell-based assay.
In this study, these analogs were designed, synthesized, and evaluated in a human cell-based assay.
A novel yeast cell-based assay was developed for the detection of estrogenic activity in wastewater.
Several of these analogs were potent VEGF inducers in a cell-based assay.
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