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Exact(14)
Cell were washed and fresh medium added.
Cell were washed twice with PBS, subjected to lysis in cell lysis buffer, and sonicated for 15 seconds.
Cell were washed, resuspended in IL-3 or G-CSF, and EGFP fluorescence was measured two days later using flow cytometry.
For Western analysis of proteins from GNPs, cell were washed twice in cold PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer [42] supplemented with a protease inhibitor cocktail (Roche, Indianapolis, IN).
Briefly, cell were washed one time with 1XPBS, fixed with 4% paraformaldhyde (PFA) for 10 min, washed 3X with PBS before permeabilizing with 0.5% Triton X (in 1XPBS) solution for 5 min at room temperature (RT).
Cell were washed three times and analyzed by FACS analysis.
Similar(46)
At first, the cell was washed with distilled water.
The cells were washed twice in PBS.
After incubation, the cells were washed three times with HBSS.
Finally, the cells were washed 3 more times and imaged.
After incubation, cells were washed with PBS and air dried.
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