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Other forms of fuel cell were then thought to be more promising.What the Ballard team brought to the story was engineering and passion.
The eight sector values for each cell were then added to give % vesicle/tubule index per cell.
Cell were then pulsed for 7 minutes with 0.5 µCi/dish [3H]deoxyglucose in DMEM containing 0.5% BSA, 25 µM HEPES and 100 µM glucose.
The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope Whole cell lysates were obtained by treating cells with RIPA buffer (1% w/w NP-40, 1% w/v sodium deoxycholate, 0.1% w/v SDS, 0.15 M NaCl, 0.01 M sodium phosphate, 2 mM EDTA, and 50 mM sodium fluoride) plus protease and phosphatase inhibitors.
Cell were then stained with Alexa Fluor 488 conjugated secondary antibody for 90 min at room temperature (RT) and examined under microscope.
The atomic positions within the distorted unit cell were then reoptimized (while fixing the z-coordinates of the lower-most hydrogens to keep the layer flat).
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The resulting cell is then induced to divide and eventually becomes an early-stage embryo.
Those cells were then implanted in mice specially bred to develop diabetes.
Human induced pluripotent (iPS) stem cells were then injected into the pig embryo.
The cells were then harvested by centrifugation.
Cells were then incubated with primary antibodies.
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CEO of Professional Science Editing for Scientists @ prosciediting.com