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Flow cell profiles were inspected as a quality gate; thus, samples that failed to have a correct positioning inside the flow cell were discarded.
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If only one cell was recovered, the remaining cell was discarded.
Non-adherent cells were discarded after 60 min.
When FCS was used for supplementation and non-adherent cells were discarded, both media were comparable.
The collected samples were centrifuged at 10,000 rpm for 5 min and cells were discarded.
Cells were discarded when the series resistance values were over 20 MΩ.
The plate was incubated at 37°C for 2 h, and the non-adherent cells were discarded.
Effects on predicted off-target sites in the CRISPR-treated cells were discarded after a targeted deep-sequencing screen.
Both media were comparable also when pooled human serum (hS) was used instead of FCS, but the numbers of hMSCs were lower when non-adherent cells were discarded.
After 5 days of culture, floating cells were discarded and attached macrophages were re-plated in 12-well plates overnight prior to stimulation.
Nonadherent cells were discarded.
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