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Each cell well is scanned about every two seconds, so a plate containing 384 wells is done within 12 to 13 minutes.
The visible-light power density at the liquid surface in cell wells was 12 mW/cm2 as measured by a power meter (PM10V1; Coherent, Santa Clara, CA, USA).
The mortality rate in the 10 cell wells was 98.8%.
Normal cell culture and no cell wells were set as control.
Control cell wells were treated with an aliquot of drug-free tissue culture medium.
Different cell wells were treated with 0, 2.5, 5 and 10 μM of CuE for 1 3 days.
For quantification of GFP-positive cells, wells were trypsinized, cells pelleted and fixed in 3.7% formalin.
For LNCaP cells, wells were coated with poly--lysine (20 μg ml−1 in water) to aid adhesion.
After attachment of the cells, wells were flooded with 0.5 mL of the same culture medium mix and experiments commenced.
Cells (1×104 cells per well) were seeded in 96-well plates.
In 96 well plates, L-929 and MCF-7 cells (10,000 cells per well) were cultured in each well.
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CEO of Professional Science Editing for Scientists @ prosciediting.com