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A summary of cumulative protein production and measurements of wet cell weight at the end of the experiment averaged across the four microbioreactors is shown in Table 2.
The optimal induction conditions to be determined consisted of inducer concentration and dry cell weight at the time of induction.
As shown in Fig. 4, during the growth phase, the maximum biomass in the fermentor reached 23.9 g/l (dry cell weight) at 48 h.
µ K ' = In w t 2 / w t 1 / t 2 > t 1 ; t 2 t 1 (1)where wt2 and wt1 are the dry cell weight at the different time points (t1 and t2), respectively.
In this study, both the total lipid content and neutral lipid content exhibited the most marked increase under N-deprivation, with 50.32% and 34.29% of dry cell weight at the end of cultivation, respectively.
The total lipid and neutral lipid contents exhibit the most marked increment under nitrogen deficiency, achieving 50.32% and 34.29% of dry cell weight at the end of cultivation, respectively.
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Both strains reached the maximum dry cell weight (DCW) at about 40 hours of propagation but the CBH protein level continued to elevate for many hours after the DCW stopped increasing.
The cultures were incubated at 30 °C for 48 h and (R -3-HB and dR -3-HB weight weR -3-HBured andintervals over the incubation periodry
Batch growth under aerobic and anaerobic conditions was conducted at 37°C and spectrophotometric measurements, viable cells/ml, and biomass (g dry cell weight) were determined at each time point.
To extend the application of the bacterial biosensor, we assembled a bioadsorption system that can trigger bacteria to sense and adsorb 13 ± 0.3 mg/L of zinc and 11.4 ± 0.42 mg/L of copper per gram of dry cell weight with induction at a concentration of 100 mg/L of the respective metal ion.
The cell mass was washed with distilled water and dry cell weight was estimated at 103 °C.
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