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In order to confirm that SWCNTs could penetrate cell wall to enter into the inner of plant cell, we used FITC-labeled SWCNTs (FCNTs) to incubate with plant cells.
To obtain more insight in the biogenesis of COX in a living cell, we used yeast as a model organism to design a way to pulse label holo-COX with green fluorescent protein (GFP).
For the ganglion cell we used the single compartment model of Fohlmeister and Miller [25].
To separate signals from fluorescent proteins in a cell, we used a linear unmixing method, as described previously [46], [47].
For statistical analysis of expression changes between PPARγ siRNA and control siRNA treated cell we used PARTEK's Genomics Suite.
To obtain the exact number of mRNA copies per cell, we used total RNA from laser-dissected hippocampal neurons.
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In HTB56 cells, we used lipofectamine reagent (Invitrogen).
To deplete mDia1 in cells, we used the RNAi technique.
For the tubular cells we used a murine proximal tubular epithelial cell line [30], [31].
In the case of stably transfected, K562 cells, we used the same protocol with Lipofectamine.
To test whether Stat3 binds to the ROR1 promoter in CLL cells, we used EMSA.
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