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For one cell we calculated a maximum 3He polarisation of 79% with a T1 of 633 h.
For each cell, we calculated FRET efficiency and distances (r) between fluorophores in each pixel of ROI.
For each cell, we calculated a position selectivity index defined as SI = (rmax−rmin)/(rmax+rmin) where rmax and rmin were maximum and minimum responses across all positions.
For the numerical simulation of noiseless cell, we calculated the ordinary differential equations, where the noise terms are omitted in Eq. (3), with the Euler method.
For each cell, we calculated a scale factor as a ratio of the cell's 18S level to the mean 18S level of its batch, representing the relative size of each cell with respect to the mean cell size.
For each 10-km grid cell, we calculated the Bray-Curtis distance or dissimilarity metric between current and future community composition, based on predicted probability of occurrence for the same 60 avian focal species.
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To set an ID number for each grid cell, we calculate the row-column number (RCN) for each grid cell in step 2. To reduce the updating range of a mobile sink's location, we introduce the direction number (DN) in step 3. 1) Calculation of side length of grid cells.
Within each cell we calculate virus levels and levels of RNA silencing proteins with a detailed model.
Briefly, we analyzed the percentage of labeled cells in ten 2,500 µm2-counting frames per brain area and animal using the 99% Poisson criteria; of the labeled cells, we calculated the average number of silver grains.
To complement our quantifications of apical cell area and gain some insight into the membrane content of the AS cells, we calculated the ratio between the real cell perimeter and the perimeter of the best-fitted ellipse (Figure 5A).
To quantify the observed Ncl/Tpt1 colocalization differences between mitotic and interphase cells we calculated three different parameters: Pearson's correlation coefficient, percentage overlap of Tpt1 and percentage overlap of Ncl or Ncl-P.
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