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Exact(14)
At first, the cell was washed with distilled water.
At the beginning of the experiment, the measuring cell was washed with the working buffer solution (PBS), to obtain a stable signal of the device the baseline.
For regeneration of the bioselective element (a destruction of bonds between the immobilized protein A and IgG as well as a removal of the latter), 120 μl of 40 mM citrate buffer (pH 2.5) was injected into the measuring cell, then the cell was washed with PBS until stabilization of the sensor signal [27].
The flow cell was washed with degassed "actin buffer" (25 mM KCl, 25 mM Imidazole, 1 mM EGTA, 4 mM MgCl2, pH 7.4) after a one minute incubation.
After a one minute incubation with donor, the flow cell was washed with degassed low salt buffer and blocked with 0.5% (v/v) Tween 20 in degassed low salt buffer for myosin, or 2% PVP40 in degassed low salt buffer for HMM for 1 min. TRITC-phalloidin labeled actin filaments were introduced, followed by two degassed actin buffer washes.
Deposition cell was washed and suspended with 50 mM phosphate buffers (pH 7.2).
Similar(46)
Cell were washed and fresh medium added.
The plate containing viable cells was washed twice with PBS.
The harvested cells were washed and lyophilized.
And cells were washed to remove media.
The cells were washed with PBS, fixed, and washed again.
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